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Hormonal data did not show adrenal insufficiency (details are reported in Table 1 ). 3.2. DNA Sequencing. Primer Sequence HSD3B2_1-2_FW GCTCCAGTCCTTCCTCCAGG HSD3B2_1-2_REV AGGTCAACCTCCCCACACCC HSD3B2_3_FW GGATGTGTGACAATTCACTGC HSD3B2_3_REV TCTTTCTGATCCTCATTTAACCAA HSD3B2_4_FW CATGTGGTTGCAGCTCCTTT HSD3B2_4_REV GAAGAAGACAGTAAGTTGGG HSD3B2_4INT_FW * ACCTTGTACACTTGTGC HSD3B2_4INT_REV * TGTGGCGGTTGAAGGG. 3.3. In Silico Analysis. With respect to the missense mutation, the evaluation of the risk of pathogenicity was thus the result of the following individual tools integration: MaxEntScan; Sequences from Mutation Surveyor v3.30 reporting the mutations found in Patient n.1, Patient n.2 and their parents.

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In the present study, we systematically idntified 19 putative β -hsd genes in the E. coioides genome, and subsequently characterized the genes in terms of position in the genome, exon-intron structure and conserved domains. System Bet – comprises more than one betwinner 365 tips accumulator bet of the same size. Finally, an analysis of grouper β -hsd gene expression levels during sex reversal indicated that these genes may play important roles in this significant physiological process. Our study represented the first comprehensive investigation of the fish β -hsd gene family, and the resulting data will undoubtedly be useful for future analyses, including further research into the role of β -hsd genes and sex steroids in sex reversal of grouper. In the gonad, two ( hsd3b1 and hsd17b14 ) and eight ( hsd3b7 , hsd17b1 , hsd17b7l , hsd17b10 , hsd17b12a , hsd17b12b , hsdl1 , and hsdl2 ) β -HSD genes were up- and downregulated during sex reversal, respectively. O'que é pixbet.

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