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BetAmo velez sarsfield x patronato Nederland. Cortical neurons from knockout embryos showed an almost complete loss of Arhgef7 expression (Fig. 3b). 75 + 5% of the neurons from homozygous Arhgef7-cKO embryos were unpolarized and did not extend a Tau-1 positive axon compared to 17 ± 3% in cultures from heterozygous embryos (Fig. 3c). Arhgef7 is required for axon formation in the cortex. ( a ) The expression of Arhgef7 in the cortex from Arhgef7 fl/+ ; Emx1 Cre/+ (+/−) or Arhgef 7 fl/fl ; Emx1 Cre/+ (−/−) E17.5 embryos was analyzed by Western blot. Analysis of GAPDH expression confirmed that the loading of comparable amounts of protein. Numbers indicate the molecular weight in kDa. ( b ) Cultured neurons from the cortex of Arhgef7 fl/+ ; Emx1 Cre/+ (+/−) or Arhgef 7 fl/fl ; Emx1 Cre/+ (−/−) E17.5 embryos were stained at 3 d.i.v. (stage 3) with an anti-Arhgef7 (green), an anti-MAP2 (red) and the Tau-1 (blue) antibody. ( c ) The formation of axons was analyzed at 3 d.i.v. Troca de titularidade vivo.Hellforge Relic. Anvil of Annihilation.
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