In addition, we also identified 24, 16, 24, 17, 14, 19, 19, 19, 17, and 27 β -hsd genes in mouse, chicken, xenopus, coelacanth, spotted gar, fugu, tilapia, medaka, stickleback, and common carp, respectively (Table 1). The gene and protein IDs of the β -hsd genes of all species are listed in the attached table (Supplementary Table S1). Figure 1. Chromosome localization of 19 β -hsd genes from orange-spotted grouper. The exon-intron structure of the grouper β -hsd genes was further characterized, and showed that the number of introns in each β -hsd gene varied from 2–16 (Figure 2B). Hsd3b1 was the gene with the lowest number of introns, containing only two, while hsd17b4 had the greatest amount at 16. In addition, a phylogenetic tree was constructed using the grouper β-HSD protein sequences to determine whether the exon-intron structure is consistent with the phylogenetic tree (Figure 2A).
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