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Cdc42 was used as a positive control (Suppl. Fig. Como o nome sugere, depois betandyou que es um ataque cassino pano do jogo roletada Gil Alcalá. While little active TC10 was detectable without co-expression of a GEF, a strong signal was apparent upon co-expression with Arhgef7 (Fig. 5e). The addition of a phosphatase inhibitor increased the amount of active TC10 consistent with reports that Arhgef7 activity is regulated by phosphorylation 10,31,55 . These results show that the co-expression of Arhgef7 increased the amount of GTP-bound TC10 indicating that it acts upstream of it. Several TC10 effectors are known that could mediate its function in axon formation. These include Par6, Pak1, N-WASP and Exo70 1,53,58,59,60 . Arhgef7 has been implicated so far mainly in the regulation of the actin cytoskeleton 42,58 . In addition to changes in cytoskeletal dynamics, the expansion of the plasma membrane (PM) by the insertion of specialized vesicles is essential for axon formation 61,62 . The exocytosis of these specialized plasmalemmal precursor vesicles (PPVs) in the growth cone requires the exocyst complex, a conserved octameric complex that mediates the tethering of vesicles at the PM prior to their fusion 49,63 .
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