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17, 79–85. doi: 10.12717/DR.2013.17.2.079. Keywords : β-HSD, orange-spotted grouper ( Epinephelus coioides ), genome-wide analysis, gene expression, sex reversal. 3β-HSD is responsible for the conversion of pregnenolone to progesterone, 17β-hydroxypregnenolone to 17β-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to androstenedione and androstenediol to testosterone. 3β-HSD is encoded by two closely related genes located on chromosome 1 (1p13.1), HSD3B2 and HSD3B1, coding for type II and I isozymes, respectively. Type I isozyme (HSD3B1) is mainly expressed in the placenta and peripheral tissues, and type II isozyme (HSD3B2) is mainly expressed in the adrenal gland and gonads [4,5]. Patient n.2 was the older brother of patient n.1. At the time of clinical and biochemical screening, they were 2.5 years old. They were a full-term newborn (gestational age = 40 weeks), born after an uneventful pregnancy by vaginal delivery. Their weight at birth was 3110 g and their length was 51.5 cm. Their Apgar scores were 10 and 10 at 1 and 5 min, respectively. Neurological exam was normal. Neither asthenia nor other health problems were reported.
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