Furthermore Hsd17b1 converts inactive estrone to active estradiol (Mindnich et al., 2004), Hsd17b3 is an essential enzyme for the synthesis of 11-KT (Mindnich et al., 2005) and Hsd20b2 converts 17alpha-hydroxyprogesterone to 17alpha,20beta-DP, which has been identified as the maturation-inducing hormone of several teleosts (Senthilkumaran et al., 2002). Materials and Methods. Genome-Wide Identification of β -hsd Genes. Phylogenetic Analysis. Gene Expression Profiling by Quantitative Real-Time PCR (qRT-PCR) A total of 22 and 19 β -hsd genes were collected from the human and zebrafish genome, respectively, which were used to construct HMM models. A set of 19 putative members of the β -hsd genes have been identified from the orange-spotted grouper genome (Table 1). Among the 19 β -hsd genes, a total of 14 were distributed across 10 linkage groups (LGs) in the grouper genome (Figure 1). In addition, we also identified 24, 16, 24, 17, 14, 19, 19, 19, 17, and 27 β -hsd genes in mouse, chicken, xenopus, coelacanth, spotted gar, fugu, tilapia, medaka, stickleback, and common carp, respectively (Table 1).
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