Todo o dinheiro já era. Eu quero trabalhar para poder pagar minhas contas. Já estou devendo”, declarou ao jornal Folha de S. Paulo . Dourado também teria usado o dinheiro para ajudar seus familiares e investido em uma marca própria de roupas e acessórios. Rodrigo Cowboy , campeão do BBB2: Alega que o prêmio foi perdido ao investir em negócios que não deram certo. Ele conta que gastou o prêmio que ganhou -- foram R$ 500 mil -- comprando mil bezerros.
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Introduction. 2.2. Immunohistochemical Staining. 2.4. Experimental Scoring System. Quantitative ER score = 1/20 * (percent of ER-positive cancer cell nuclei + (average nuclear intensity × 100)). Both the semi-quantitative and quantitative analyses of the 66 cases of ILC considered in our study included the determination of the following three parameters: Percent of ER-positive cells, average nuclear coloration intensity, and quantitative ER score. In the semi-quantitative, classical approach, the first two parameters were evaluated by at least two experienced pathologists and were based on their subjective treatment of the coloration intensity (which attained integer values, 0–3), while the percentage of the ER-positive nuclei was assessed by manual counting and was scored typified according to the Allred ranges ( Figure 3 ). The proposed quantitative approach in this work included both the counting and coloration intensity evaluation by a software that utilized a deconvoluted color intensity and a counting grid. The results of the quantitative assessment were scaled to be compatible with the already utilized Allred score. But don’t be too shocked if pin up meaning you hear a native speaker use it. The deconvoluted color intensity assessment allowed us to detect cases where OD values of 0.1 and less were present in the negative cases ( Figure 4 ). All cases that were determined to have a higher OD of 0.1 were observable by the pathologists. Thus, we chose OD = 0.1 as the limit when the score 0 or 1 for nuclear coloration was given to a case to maintain compatibility with the Allred scoring system.
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